Actinomycin Binding Capacity in Human Leukaemic Lymphoid Cells

Cytochemistry of leukaemic lymphoid cells.

Changes in DNA capacity for actinomycin-D binding in nuclei of interphase cells.

3H-AMD binding to DNA in interphase nuclei was tested on asynchronous and synchronous LS/BL cell populations under physiological conditions and after exposure to gamma rays (60Co). 3H-AMD binding to DNA in an asynchronous cell population appeared to be nearly constant and independent of 3H-AMD concentration. However, in comparing individual cells, a great variability could be observed. In synch...

Binding to Lymphoid Cells

A receptor for fluid phase human complement (C) components C3 and C3b (1, 2) was found on the surface membranes of human lymphoblastoid cells. No other C proteins or serum factors were needed to mediate binding of C3 or C3b to this receptor. In studying its biological importance, we demonstrated that Raji cells cultured in medium containing fresh normal human serum (NHS) 1 and cobra venom facto...

Human innate lymphoid cells.

Innate lymphoid cells (ILCs) are lymphoid cells that do not express rearranged receptors and have important effector and regulatory functions in innate immunity and tissue remodeling. ILCs are categorized into 3 groups based on their distinct patterns of cytokine production and the requirement of particular transcription factors for their development and function. Group 1 ILCs (ILC1s) produce i...

Carbohydrate receptor-mediated gene transfer to human T leukaemic cells.

The mucin-type carbohydrate Tn cryptantigen (GalNAc alpha 1-O-Ser/Thr, where GalNAc is N-acetyl-D-galactosamine) is expressed in many carcinomas, in haemopoietic disorders including the Tn syndrome, and on human immunodeficiency virus (HIV) coat glycoproteins, but is not expressed on normal, differentiated cells because of the expression of a Tn-processing galactosyltransferase. Using Jurkat T ...